Effects of hypothermia within the lateral ventricle on the neural cell apoptosis after rabbit cerebral ischemia
- VernacularTitle:脑室内低温对脑局部缺血后神经元凋亡的影响
- Author:
Wei WU
;
Jixin SHI
;
Huilin CHENG
;
Guiqin SUN
;
Hongxia YIN
- Publication Type:Journal Article
- Keywords:
Ventricle trickled;
Mild hypothermia;
Cerebral ischemia-reperfusion;
Apoptosis
- From:
Journal of Medical Postgraduates
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effects of continuous trickle of low temperature liquids through the lateral ventricle on neural cell apoptosis after rabbit local cerebral ischemia/reperfusion. Methods:The middle cerebral artery (MCA) of New Zealand rabbit was clipped by micro aneurysm clip for 2 hours and reperfused for 24 hours. Immediately after clipping the MCA, we trickled the left lateral ventricle continuously with low temperature liquids(22℃) to decrease the brain temperature to mild hypothermia (33℃-35℃)and maintained for 2 hours. After reperfusion for 24 hours , we assessed TUNEL method to determine the apoptotic cell rate in the sham-operated group, the control group and the mild hypothermia group respectively. Results:The apoptotic cell rate of the cortex tissues accommodated by MCA in the mild hypothermia group was obviously lower than that in the control group(P0.05). Conclusion:Trickling ventricle with low temperature liquids could decrease the apoptotic cell rate and alleviate cerebral ischemia/reperfusion injury.
