Propylene glycol mannate sulfate down-regulated the expression of adhesion molecules(ICAM-1,VCAM-1)in kidney of diabetic rats
- VernacularTitle:甘糖酯降低实验性糖尿病大鼠肾脏ICAM-1,VCAM-1表达?
- Author:
Ruxia WANG
;
Jianting HU
;
Ling GAO
;
Jiajun ZHAO
;
Xiume ZHANG
- Publication Type:Journal Article
- Keywords:
propylene glycol mannate sulfate;
adhesion molecule;
diabetic nephropathy;
microalbuminuria
- From:
Chinese Journal of Marine Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of propylene glycol mannate sulfate(PGMS) on the expression of ICAM-1 and VCAM-1 in kidney of diabetic rats, and explore the protective effect of PGMS on kidney. Methods Diabetes was induced by injecting STZ. Then the rats were randomly divided into four groups: normal control group, diabetic modal group, treatment group with high-dose and low-dose of PGMS. 8 weeks later, the blood glucose concentrations, HbA1c, the ratio of kidney weight to body weight, urinary albumin excretion rate (UAE ) in 24 hours were examined. The ICAM-1 and VCAM-1 located were detected by immunohistochemistry, while the expression of ICAM-1 and VCAM-1 were detected by Western blot method and the levels of ICAM-1 and VCAM-1 mRNA by RT-PCR.Results PGMS could reduce kidney hypertrophy and lower urinary albumin excretion rate (UAE)of 24-hour significantly. Immunohistochemical study with light microscopy demonstrated that the levels, both ICAM-1 and VCAM-1 in the kidney of test groups decreased obviously. PGMS down-regulated the mRNA and protein levels of ICAM-1 and VCAM-1 significantly . A positive correlation between expression of ICAM-1,VCAM-1 and kidney lesion was observed. Conclusion: PGMS decreased the expression of ICAM-1 and VCAM-1 in diabetic rats.
