Determination of adenosine in Ligia exotica with ultraviolet spectrophotometry
- VernacularTitle:紫外分光光度法测定海蟑螂中腺苷含量
- Author:
Weidong XUAN
;
Jun BIAN
;
Huawu ZENG
;
Jing XEAO
- Publication Type:Journal Article
- Keywords:
Ligia exotica;
adenosine;
ultraviolet spectrophotometry;
assay
- From:
Chinese Journal of Marine Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a UV method of determination of adenosine in Ligia exotica. Methods Adenosine was extracted with 37℃ water and separated by TLC silica gel, the absorbance was detected at 260nm with UV spectrophotometry.Results The cabibration curve for adenosion was linear within the range of 1.0~20.0?g?mL -1, r=0.9998 (n=6), the average recovery was 99.3%,RSD=1.9% (n=3). Conclusion The method was simple, convenient and accurate for determination of adenosine in Ligia exotica.
