Inhibition of survivin and bcl-2 Antisense Oligodeoxynucleotides Combined Transfection on the Human Gallbladder Carcinoma Cell Line GBC-SD in vitro
- VernacularTitle:单独及联合转染survivin和bcl-2反义寡核苷酸诱导胆囊癌细胞凋亡的研究
- Author:
Limin FENG
;
Jianli WANG
;
Xinlin WU
;
Xihong JIANG
;
Nanhai SHOU
- Publication Type:Journal Article
- Keywords:
gallbladder carcinoma;
survivin;
bcl-2;
oligonucleotide;
antisense;
transfection
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of survivin and bcl-2 antisense oligodeoxynucleotides ( AsODN) combined transfection on the human gallbladder carcinoma cell line GBC-SD in vitro. Methods: Survivin and Bcl-2 protein expressions were detected by immunohistochemical method; Cultured cells were divided into 4 groups: Nomal control group, survivin antisense observed group, bcl-2 antisense observed group and combined group. After transfected for 24 h, the expression of survivin mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). Cell morphological changes were observed under electron microscopy. Apoptosis index (AI) was examined by flow cytometry; Inhibitory rate (IR) was determined by the colorimetri MTT cell viability and proliferation assay. Results: Survivin and Bcl-2 protein were highly expressed in gall bladder carcinoma cells; The expression of survivin mRNA was decreased 47. 8%. Abnormal morphological changes of cells were observed in the three AsODN transfection groups; The AI in survivin antisense observed group,bcl-2 antisense observed group,and combined group was 11.38%?3.91% , 9.26%?4.15% , 28.45%?6.34% respectively and significantly higher than the nomal control group (P
