The Inhibition of Antisense TRPl in the Proliferation of Malignant Melanoma Cells in vivo and in vitro
- VernacularTitle:TRP-1反义核酸抑制恶性黑素瘤细胞增殖的体外及体内研究
- Author:
Chunying LI
;
Tianwen GAO
;
Xianlong QI
;
Gang WANG
;
Yufeng LIU
- Publication Type:Journal Article
- Keywords:
tyrosinase related protein 1, antisense;
malignant melanoma;
preliferation
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the inhibition of antisense TRP1 on cell growth of malignant melanoma(MM) and explore a new way for therapy of melanoma. Methods: Antisense TRP-1 recombinant vector was constructed and transfected into MM cells. According to the results of MTT, cell growth curves were drawn and then clonogenic assay was performed in vitro. At last, tumorigenesis assay was undertaken in nude mice in vivo. Results: Cell proliferations of TRP-1 transfected MM cells were inhibited compared with the control cells. The results of clonogenic assay displayed the difference of clonogenic percentage between TRP-1 transfected MM cells (52% , P
