PSD_(93) knockout decreased neurotoxity induced by pletelet activitaing factor
- VernacularTitle:突触后密度蛋白93基因敲除抑制血小板活化因子性神经元损害
- Author:
Yun XU
;
Rong HUANG
;
Dening GUAN
- Publication Type:Journal Article
- Keywords:
platelet activating factor;
PSD 93;
knockout;
neuron;
apoptosis
- From:
Journal of Clinical Neurology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether PSD 93 reduce neurotoxity induced by platelet activating factor (PAF) and study on the mechanism of cell molecularbiology.Methods PAF treated the cultural cortex neurons in wild type and PSD 93 knockout mice and the cells were stained with PI/Calcein AM for apoptosis; neuronal viability was measured by MTT. The expression of PSD93 and PSD95 protein in neurons was tested by Western Blot.Results PSD 93 and PSD 95 expressed in wild type neurons and only PSD 95 expressed in PSD 93 knockout. Cortex neuronal apoptosis in PSD 93 knockout mice was lower then that in wild type ( P
