The role of dephosphorylated RB protein in human breast cancer cell apoptosis
- VernacularTitle:去磷酸化RB蛋白在乳腺癌细胞凋亡中的作用
- Author:
Wenwu WANG
- Publication Type:Journal Article
- Keywords:
Dephosphorylated RB protein;
Breast cancer;
Apoptosis;
Adriamycin
- From:
Journal of Medical Postgraduates
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the role of the expression of dephosphorylated RB protein for cell apoptosis in human breast cancer. Methods: In our works, human breast cell lines (MCF-7/S,the chemosensitive cell line and MCF-7/Adr, the chemoresistent cell line)were evaluated. The expressive levels of dephosphorylated RB protein were detected with immunocytochemistry. Apoptosis rates were determined by flow cytometry(FCM). Results: Before treatment of ADR, phosphorylated RB protein was positive in two cell lines, but dephosphorylated RB protein was negative; After treatment of different concentration ADR, when the concentration of ADR was increased, expression of dephosphorylated RB protein elevated accordingly in MCF-7/S, but no significant change in MCF-7/Adr. Apoptosis and cycle was detected by FCM assays shows ADR induced apoptosis of MCF-7/S more than MCF-7/Adr(P