The law of HSP70 expression and significance in Vero-E6 cells induced by hantavirus
- VernacularTitle:汉坦病毒诱导非洲绿猴肾上皮细胞热休克蛋白70的表达规律及意义
- Author:
Lu YU
;
Heng MA
;
Jun ZHU
;
Shoujin YANG
- Publication Type:Journal Article
- Keywords:
Hantavirus;
Heat shock protein 70;
Stress response
- From:
Journal of Medical Postgraduates
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the law and significance of stress response after Vero-E6 cells infected with Hantavirus (HTV). Methods:The law of expression of HSP 70 and its mRNA level were detected by immunocytochemical staining, laser confocal microscope and RT-PCR. Results:After being infected with HTV, immunocytochemical staining exhibited that the expression of HSP 70 increased. laser confocal microscope and RT-PCR detection showed that the level of HSP 70 and its mRNA increased quickly and kept at a high level in comparison with the normal control(P