Genistein inhibited hypoxia inducible factor-1? expression induced by cobalt chloride in human retinal pigment epithelium cells
- VernacularTitle:金雀异黄素抑制氯化钴诱导的人视网膜色素上皮细胞缺氧诱导因子1?表达的研究
- Author:
Hua LI
;
Hong YAN
;
Jinshun PAN
;
Bin WANG
- Publication Type:Journal Article
- Keywords:
Genistein;
Hypoxia-inducible factor-1?;
Cobalt chloride;
Human retinal pigment epithelia cells
- From:
Journal of Medical Postgraduates
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The time-dependent changes of hypoxia-inducible factor-1? (HIF-1?) expression induced by CoCl_(2 ) treatment and the effects of genistein on the level of HIF-1? expression in human retinal pigment epithelium cells were studied. Methods:Using a confocal scanning laser microscope coupled to a computer, HIF-1? expression was determined. Results:CoCl_(2 ) treatment could significantly elevate the level of HIF-1? expression. At 0.5 hour after CoCl_(2 ) treatment, the highest level was observed. Genistein 50 ?mol/L, 100 ?mol/L, 200 ?mol/L could suppress HIF-1? expression in a concentration-dependent manner. Conclusions:These results suggested that genistein could inhibit HIF-1? expression induced by CoCl_(2).
