Qualitative and quantitative analysis of glycosaminoglycan extracted from scallop skirts by gas chromatography and infrared spectrometry
- VernacularTitle:扇贝裙边中糖胺聚糖的气相色谱定性定量分析及红外光谱分析
- Author:
Shan LI
;
Sai LIU
- Publication Type:Journal Article
- Keywords:
scallop;
Chlamys farreri;
glycosaminoglycan;
neutral monosaccharides;
gas chromatography;
infrared spectrometry
- From:
Chinese Journal of Marine Drugs
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the chemical constituents and structures of the glycosaminogly-cans isolated from the leftover bits of the Chlamys farreri and Argopecten irradisus. Methods The qualitative and quantitative analyses of monosaccharides obtained by alcoholyses with HCl-methylalcohol from the samples were done by gas chromatography with standard contrast and inner standard methods. Mixed with KBr and pressed into pellet, the samples were analyzed by infrared spectrometer scanning from 4000-500 cm-1. Results The contents of monosaccharides from the samples were rhamnose 0. 75%, xylose 0. 63%, fucose 0. 67%, mannose 0. 97%, glucose 1. 11% and galactose 1. 59%, respectively. The infrared spectrum showed that the samples had typical infrared spectra of glycosaminoglycan. Conclusions The glycosaminoglycan isolated from the leftover bits of the Chlamys farreri contains neutral monosaccharides. Compared with standard glycosaminoglycans, the infrared spectra of the two samples are similar to that of hyaluronic acid.
