The Study of the Susceptibility of M.tuberculosis to Rifampin by Micro-well Phage Replication Assay
- VernacularTitle:应用微孔噬菌体扩增法检测结核分枝杆菌对利福平的敏感性
- Author:
Xuxia ZHANG
;
Hairong HUANG
;
Chuanyou LI
;
Weimin LI
;
Zhongquan LIU
;
Yu MA
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Bacteriophages;
Rifampin;
Antitubercular agents
- From:
Chinese Journal of Laboratory Medicine
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess susceptibility of to 173 clinical M.tuberculosis isolates to by Micro-well Phage Replication Assay(MPRA).Methods To prepare the isolates and expose them to rifampin . To prepare phage D29 suspension . MPRA array for the susceptibility to rifampin.Results Compared with the absolute concentration method ,there were the same result of 38 isolate in 42 susceptible strains and of 124 isolate in 131 resistant strains. Between result of MPRA assay and absolute concentrstion (method,) concordance was 93.6%(38/42), susceptibility was 94.9%(124/131) and specificity is 90.5%((38/42).)Conclusion MPRA assay is a good and rapid method for drug susceptibility of rifampin.
