Development of excitotoxicity model with primary hippocampal neurons and implications of viability essay
- VernacularTitle:原代海马神经元兴奋毒性模型的建立及存活率的检测与意义
- Author:
Jie SHEN
;
Changkai SUN
;
Ming FAN
;
Hui MA
;
Ping LIN
;
Yuying TONG
;
Guangxia SHI
- Publication Type:Journal Article
- Keywords:
Neurons;
Glutamate;
Models, animal
- From:
Chinese Journal of Laboratory Medicine
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the injury and protective action of drugs on neurons, the model of glutamate excitotoxicity on primary cultured hippocampual neurons from new born rats were( set up. Methods)By use of trypan blue dye staning and testing the lactate dehydrogenase leakage from cultured neurons, to investigate the neuron survival rate. Results We found the injury of neurons was related with the concentration of glutamate. NMDAR non-competitive antagonist —MK-801 could protect the glutamate excitotoxic damage on neurons. Conclusion The glutamate results in neuron injury through NMDAR; the model of neuron culture was sufficient for glutamate-induced excitotoxicity.
