Prepae B-lymphoblastoid cell lines of HLA novel allele B ~*5610 in a family
- VernacularTitle:HLA新基因B"非汉字符号"610携带者家系B淋巴细胞系的建立
- Author:
Zhen LI
;
Hongyan ZOU
;
Guoguang WU
- Publication Type:Journal Article
- Keywords:
HLA (Human leukocyte antigen;
EBV (Epstein-Barr virus) FBS (fetal bovine serum);
CsA (CyclosporinA)
- From:
Chinese Journal of Blood Transfusion
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare B-lymphoblastoid cell lines of HLA novel allele B*5610 in a family for further study and identification . Method Isolate mononuclear cells under aseptic conditions from the peripheral blood. After infection with Epstein-Barr virus, the cells were cultured in 20% FBS, 2?g/ml CsA RPMI 1640. Results Immortalized B-lymphoblastoid cell lines of five B *5610 carriers in a family were achieved, and the new genes were inherited stably. Conclusion Our work is important for storing and breeding the precious material of biomedicine because the B *5610 genes in the immortalized B-lymphoblastoid cell lines were inherited stably.
