The study of cyclin A antisense oligodeoxynucleotides on proliferation regulation of K562 cell
- VernacularTitle:细胞周期素A的特异反义脱氧寡核苷酸对K562细胞增殖的调控作用
- Author:
Cunrong JIA
;
Shirong XU
;
Jie MA
- Publication Type:Journal Article
- Keywords:
Cyclin A;
Antisense oligodeoxynucleotide;
Leukemia
- From:
Chinese Journal of Blood Transfusion
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cyclin A antisense oligodeoxynucleotide (ASON) on mediating proliferation of K562 cell. Method After in vitro co-culture of cyclin A ASON with K562 cell, cyclin A protein expression levels were measured by flow cytometry. Cell growth was detected by trypan blue dye exclusion and colony-forming experiment. Results In cyclin A ASON group, cyclin A protein expression was significantly inhibited, compared to those in sense oligodeoxynucleotide (SON) group and blank group. Moreover, when the ASON concentration increased, the proliferation ratio of K562 cells and the CFU- K562 were significantly inhibited. Conclusion Cyclin A ASON can specifically inhibit cyclin A protein expression as well as inhibit the K562 cell proliferation and can lead to leukemic cell apoptosis. The effect of cyclin A ASON is concentration dependent.
