Overexpression of p27 gene inhibits the neointimal formation after balloon injury in rabbit
- VernacularTitle:过表达p27基因抑制血管损伤后新生内膜的形成
- Author:
Qingping TIAN
;
Yong HUO
;
Yanhong GUO
- Publication Type:Journal Article
- Keywords:
Genes, p27;
Tunica intima
- From:
Chinese Journal of Interventional Cardiology
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the function of p27 gene on the neointimal formation after balloon injury in rabbit carotid artery. Methods The cultured vascular smooth muscle cells (VSMCs) were infected with Adp27. Using MTT assay we measured the proliferation of VSMCs. Balloon injured rabbit carotid arteries were also infected with Adp27, and then we evaluated the ratio of intima to media. Results MTT assay of rabbit VSMCs proliferation demonstrated an obvious inhibition in Adp27 treated group. And p27 overexpression had an effect on the cell cycle. It could induce cell cycle arrest in G1 phase and reduce the cells in S phase. Overexpression of p27 could also reduce the neointimal hyperplasia by 27.43%. Conclusion The p27 gene can reduce the neointimal formation after balloon injury in rabbit carotid artery effectively.
