An antisense oligonucleotide inhibits the expression of PDGF-? mRNA and the proliferation of rabbit vascular smooth muscle cells
- VernacularTitle:反义寡核苷酸抑制兔血管平滑肌细胞增殖和血小板源性生长因子?mRNA的表达
- Author:
Weimin YI
;
Jun JI
;
Wenping LING
- Publication Type:Journal Article
- Keywords:
Oligonucleotides, antisense;
Vascular smooth muscle cells;
Proliferating cell nuclear antigen;
Platelet-derived growth factor ? chain
- From:
Chinese Journal of Interventional Cardiology
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To use the antisense of Platelet-derived growth factor ? chain (PDGF-?) gene to inhibit the proliferation of rabbit vascular smooth muscle cells(VSMCs) in order to provide in situ basis on prevention and treatment of human restenosis. Methods A rabbit restenotic vascular model was constrcted and an antisense designed according to PDGF-? cDNA sequence as a drug was used to observe its effects on the expression of proliferating cell nuclear antigen and in situ expression of platele-derived growth factor ? chain by the VSMCs and the formation of neointima after injury. Results The antisense could significantly inhibit the expression of proliferating cell nuclear antigen and downregulate in situ expression of PDGF-? mRNA by intimal VSMCs one week after injury with inhibitory rates of 93.44% and 88.40%, respectively. Conclusion The antisense designed could inhibit the formation of neointima after injury through downregulating the expression of PDGF-? mRNA by local VSMCs, which provides the experimental basis of the antisense for the prevention and treatment the human restenosis.
