Clonging and Expression of Recombinant Human Vasostatin' s Domain and Its Ability to Inhibit Angiogenesis in CAM
- VernacularTitle:人血管抑制因子Vasostatin短片段(120-180 aa)的克隆、表达及功能研究
- Author:
Guangyu LI
;
Bin FAN
;
Yazhen WU
;
Jiaxiang WU
- Publication Type:Journal Article
- Keywords:
vasostatin;
gene expression;
angiogenesis
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To clone and express the recombinant human Vasostatin120-180aa domain and to investigate its activity of inhibiting angiogenesis in CAM. Methods: After amplifying gene of human Vasostatin120-180aa domain, we sub-cloned it into pQE30 vector and expressed Vasostatin120-180aa domain by E. coli. We also tested its ability of inhibiting angiogenesis in CAM. Results: The total gene length of human Vasostatin120-180aa domain is 180 bp. Expressed by pQE30 system in E. coli and purified by IMAC, Vasostatin120-180aa was detected by SDS-PAGE, in which there is a positive band and molecular weight is about 8 kD. Conclusions: Recombinant human Vasostatin120-180aa could play effective role in anti-angiogenesis in CAM and it showed a dose dependent effect in some degree.
