Effects of glycosaminoglycan extracted from Pinctada martensii on osteoblast proliferation , differentiation and mineralization in vitro
- VernacularTitle:马氏珠母贝糖胺聚糖对体外成骨细胞增殖、分化及矿化功能的影响
- Author:
Chunmei AI
;
Liao CUI
;
Yiyu LIU
;
Xiaoling LEI
- Publication Type:Journal Article
- Keywords:
Pinctada martensii;
glycosaminoglycan extract;
osteoblast;
proliferation;
diffe-rentiation;
mineralization
- From:
Chinese Journal of Marine Drugs
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of glycosaminoglycan extracted from Pinctada martensii on the proliferation, differentiation and mineralization of newborn rat calvarium osteoblast in vitro. Methods MTT, PNPP and ARS methods were used to measure the cell proliferation, activity of ALP and the function of mineral nodes formation of osteoblasts cultured in vitro. Results Glycosaminoglycan at concentration of 0.008～0.5g?L -1 inhibited mildly the proliferation of osteoblast cells, however, this range of concentrations of glycosaminoglycan markedly increased the ALP activity and stimulated mineral node bone formation in osteoblast. Conclusion Glycosaminoglycan extracted from the Pinctada martensii showed stimulating effects on the differentiation and mineralization of newborn rat calvarium osteoblast in vitro, but the stimulating effect on cells proliferation was not observed.
