Evaluation of the effects of methylene blue photochemical viral inactivation of red blood cells by using animal models
- VernacularTitle:利用动物模型评估亚甲蓝光化学法红细胞病毒灭活效果
- Author:
Ping LU
;
Bing LING
;
Kaicheng QIAN
- Publication Type:Journal Article
- Keywords:
Methylene blue photochemistry;
Viral inactivation;
Beagle's dog;
Duck hepatitis B virus
- From:
Chinese Journal of Blood Transfusion
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the safety and effectis of methylene blue photochemical inactivation of virus in red blood cell (RBC) by using animal models. Methods ①The effects of improving anemia was assessed by transfusing MB P RBC to Beagle’s dog. ②Duck hepatitis B virus(DHBV)was serially diluted and added into human RBC, and then one day old ducklings were inoculated with that treated RBC by intravenous injection. Sera from ducklings were collected and tested by dot hybridization. Infectivity of DHBV (ID 50 ) in RBC was calculated before and after the treatment. Results Dog RBC treated by MB P were not hemolysed after autologous transfusion. There were no changes in serum ALT, AST, BUN, and creatinine after transfusion. The ID 50 contained in RBC changed from 10 3.35 to 10 8.35 after treatment. Conclusion MB P viral inactivation of RBC is safe and effective.
