Removal of human B-like antigen on cynomolgus monkey RBC by ?-galactosidase treatment
- VernacularTitle:?-半乳糖苷酶酶解制备通用红细胞的动物实验研究
- Author:
Qiong CAO
;
Jiongcai LAN
- Publication Type:Journal Article
- Keywords:
galactosidase;
Enzyme treatment;
Cynomolgus monkeys
- From:
Chinese Journal of Blood Transfusion
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of enzymolysis of human B like antigen by ? galactosidase treatment on the morphology and function of cynomolgus monkey red blood cell (RBC) and to evaluate the safety of the enzyme treated RBC in animal transfusion. Methods RBC with human B like antigen was treated by recombined ? galactosidase and the effect was evaluated by absorption elution test. Meanwhile, morphology and function of the treated RBC were examined and compared with pre treatment RBC. ? galactosidase treated RBC was labeled with FITC and then infused to blood group A cynomolgus monkeys. Flow cytometry was used to detect the survival of donor RBC in recipient’s blood. Blood chemistry and urinalysis of recipients were performed before and after transfusion. Results The human B like antigen of cynomolgus monkey RBC was obliterated by ? galactosidase treatment and the treated RBC maintained normal morphology and function. Survival at 24 h after transfusion was 84.6% and 68.1%. T 1/2 were 7d and 8d versus 13d in the controls. There were no change in blood chemistry and urinalysis of the recipients. Conclusion Enzymolysis of cynomolgus monkey RBC does not affect the cellular function and morphology. Transfusion of the enzyme treated human B like RBC into human A like cynomolgus monkeys is safe.
