Factors influencing the assay of ?_1-antitrypsin bioactivity
- VernacularTitle:?_1-抗胰蛋白酶生物活性测定影响因素探讨
- Author:
Cifang ZHU
;
Wei ZHU
- Publication Type:Journal Article
- Keywords:
antitrypsin;
Trypsin;
PEG;
Sucrose;
Sodium citrate;
Ethanol
- From:
Chinese Journal of Blood Transfusion
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the factors influencing the assay of ? 1 antitrypsin(? 1 AT) bioactivity. Methods Various concentrations of PEG(MW, 4000), sucrose, sodium citrate and ethanol were added before determining ? 1 AT activity. Results ? 1 AT activity increased approximately 100% compared with the controls when the PEG concentration reached 20%. Conclusion PEG concentration influences the ? 1 AT activity assay.
