Study on Quantified Lyophilized serum of Hepatitis B Virus DNA in Fluorescence PCR Detection
- VernacularTitle:乙型肝炎病毒DNA定值冻干血清用于荧光定量聚合酶链反应测定的研究
- Author:
Lunan WANG
;
Jinming LI
;
Wei DENG
;
Zhongfang WANG
- Publication Type:Journal Article
- Keywords:
Fluorescence polymerase chain reaction;
Hepatitis B virus DNA;
Standard-calibration curve
- From:
Chinese Journal of Laboratory Medicine
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study suitability of a series of lyophilized serum with definitive HBV DNA value in fluorescence quantitative COBAS Amplicor HBM kit and a sample with 106 copies/ml HBV DNA was prepared, and sent to various manufactures which would be asked to detect the samples using their own kits. Then a calibration curves from CT values of the series to the corresponding concentrations was compared with that obtained from the external standard-calibration curve with the manufactures series. Results The standard-calibration curve with the series of lyophilized serum showed an excellent correlation (
