Apoptosis of Alveolar Epithelial Cells Induced by Extraction of the Second Stage Larvae of Ascaris lumbricoides
- VernacularTitle:人蛔虫Ⅱ期幼虫提取物诱导人肺上皮细胞凋亡
- Author:
Guohua PENG
;
Keng YUAN
;
Xianmin ZHOU
;
Weidong PENG
- Publication Type:Journal Article
- Keywords:
Ascaris lumbricoides;
Cell extracts;
Epithelial cells;
Apoptosis
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To induce the apoptosis of human alveolar epithelial cells(A\-\{549\}) by the extraction of the second stage larvae of Ascaris lumbricoides and investigate the extraction concentration and inducing time related to the apoptosis. \ Methods\ Following to the results of Microculture Tetrazolium Test (MTT), five concentrations of the extraction of the second stage larvae were chosen to induce the apoptosis of A\-\{549\} cells. Meanwhile, control groups without the inducement were set up. For each group, observation was made at five time points since the start of inducement, to assess the existence of apoptosis and percentage of cells showing characteristics of apoptosis. HE stain and diphenylamine reaction methods were used to assess the cell apoptosis. Agarose gel electrophoresis of DNA and flow cytometry were also employed to confirm the apoptosis for some groups. \{\ Results \ \}Observations indicated that the apoptosis ratio of A\-\{549\} cells induced by the extraction at different concentrations were significantly higher than that of the control cells (P
