The in Silico Elongation and Analysis of the EST from Schistosoma japonicum
- VernacularTitle:日本血吸虫EST序列的电子延伸及结果分析
- Author:
Hanteng LIU
;
Zhongdao WU
;
Saide ZOU
;
Xiao SHAO
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Expressed sequence tags (EST);
In silico elongation;
Sequence analysis;
Computational biology
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a platform for in silico elongation and batch analysis of Schistosoma japonicum(Sj) ESTs, acquire the potential novel genes and research the expression profile of the genes. \ Methods\ On the basis of Linux operating system and local ESTs database of Sj, the BLAST and PHRAP softwares were used to construct a program to achieve the elongation of ESTs. Stand\|alone BLAST search against the nr database helped analyze the elongated sequence. After finishing the batch analysis script, the platform was used to research the Sj gene expression profile and acquire the potential novel genes. \ Results \ The platform showed satisfactory efficiency and fidelity. 487 elongated sequences obtained from 552 and 307 elongated sequences showed high homology within the nr database downloaded from NCBI. Furthermore, 104 elongated sequences displayed significant homology but showed no homology before elongated. 27 potential novel genes were filtered out. \ Conclusion \ An effective platform for Sj ESTs data mining was accomplished and further information on the potential novel genes was acquired.
