Random Amplified Polymorphic DNA Analysis of the Genomes Among 7 Species of Ticks
- VernacularTitle:七种媒介硬蜱基因组随机扩增多态性DNA分析
- Author:
Yinshu YANG
;
Hongbin ZHAO
;
Jijun ZHANG
;
Zhiyong SHI
- Publication Type:Journal Article
- Keywords:
Ticks;
Genome;
Random amplified polymorphic DNA technique(RAPD);
Variation (Genetics);
Cluster analysis
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study genomic polymorphic DNA and genetic distance of 7 species of ticks.\ Methods\ Ticks used in this study were Dermacentor nuttalli, D.silvarum, Haemaphysalis qinghaiensis, H.formosensis, H.punctata, Amblyomma testudinarium, and Ixodes ovatus. DNA extracts of the 7 species of ticks were amplified by random amplified polymorphic DNA (RAPD) and PCR technique using 5 primers with different arbitrary single chain polynucleotide sequences. DNA fingerprint maps were analyzed and the genetic distance among 7 species of ticks were counted. \ Results \ The amplified products of the 7 species of ticks by RAPD all showed their specific DNA band. The average genetic distance among them was 0\^71. Conclusion RAPD can differentiate the 7 species of ticks.
