A novel O~1 variant allele at the ABO blood group locus
- VernacularTitle:ABO血型中的一个新变异O~1等位基因的鉴定
- Author:
Zhihui DENG
;
Qiong YU
;
Guoguang WU
;
Yanlian LIAN
;
Yuqing SU
;
Tianli WEI
- Publication Type:Journal Article
- Keywords:
ABO blood-group system;
Blood groups
- From:
Chinese Journal of Laboratory Medicine
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify novel ABO allele in Chinese population. Methods The ABO blood group was tested by serological method, and then genotyped by sequence-specific primer (PCR-SSP) , gene cloning and sequence analysis. Results A healthy blood dornor who was diagnosed as having A2 subgroup and A2O1genotype was subjected to ABO gene cloning and sequence analysis. The haplotype-specific sequence analysis indicate that two single-base deletions, where G-deletion at nucleotide position 261 and A-deletion at nucleotide position 496 were determined in the O1 allele. The nucleotide sequence of the novelO1 allele were identical to ABO 0101 allele except for A-deletion at nucleotide position 496 in exon7 of ABO locus. Conclusion We defined this 0 allele as a novel O1 variant allele, and its registered number by GenBank is AY374123.
