Protective effect of epigallocatechin-3-gallate against myocardial ischemia and reperfusion injury in rats
- VernacularTitle:表没食子儿茶素没食子酸酯对大鼠心肌缺血再灌注损伤保护作用的实验研究
- Author:
Shuanli XIN
;
Chao CHANG
;
Xiufeng ZHAO
;
Yuqin WANG
;
Gang YANG
;
Yibai FENG
- Publication Type:Journal Article
- Keywords:
Epigallocatechin-3-gallate;
Apoptosis;
Isehemia and reperfusion;
Caspase-3
- From:
Chinese Journal of Primary Medicine and Pharmacy
2008;15(8):1309-1311,插2
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect and mechanism of epigallocatechin-3-gallate against myocardial ischemia and reperfusion injury in rats.Methods The Sprague-Dawley rats underwent 30min of left anterior descending(LAD)coronary occlusion and 6 hours reperfusion to make ischemia/repefusion(I/R)injury model in vivo.Sixty male rats were randomly divided into 3 groups:sham group,I/R group,epigallocatechin-3-gallate group.Creatine kianse isoenzyme-MB(CK-MB)and the activity of Caspase-3 and the apoptotie index(AI)by TUNEL staining were measured in each group,I/R and EGCG group were measured the infarcted size(IS/AAR%).In addition,pathologic changes of myoeardial tissue were observed under electron microscopy.Results Compared with I/Rgroup,EGCG group markedly decreasedthe activity of CK-MB in serum[(951.57±123.71)vs(1826.38±205.32),P＜0.01]and the activity of Caspase-3 in myocardiaI tissue[(0.56±0.17)vs(0.81±0.20),P＜0.01],the value of IS/AAR% in EGCG group was lower than that in I/R group[(26.73±5.22)vs(41.56±6.81),P＜0.01].AI were significantly decreased in EGCG group compared with I/R group[(7.39±2.43)vs(15.62±4.28),P＜0.01].The electron microscopic examination showed that pathologic changes of myocardiocytes in the EGCG group were significantly milder than that of the I/R group.Conclusion Epigallocatechin-3-gallate has protective effect against myocardial ischemia and reperfusion injury in rats,and the protective mechanism may be related to decreasing the cardiomyocytes apoptosis by inhibition the activity of Caspase-3.
