Transcriptional expression and promoter hypermethylation of RAS association domain family 1A gene in glioma
- VernacularTitle:胶质瘤患者RASSF1A基因转录表达和启动子区甲基化的研究
- Author:
Yunxia GAO
;
Ming GUAN
;
Wanzhong ZHANG
;
Yuan LU
- Publication Type:Journal Article
- Keywords:
Glioma;
DNA methylation;
Polymerase chain reaction;
RAS association domain family 1A gene
- From:
Chinese Journal of Laboratory Medicine
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
0. 05). In glioma, the expression level was higher in glioblastornathan in oligodendroglial tumors (P = 0. 011). While the frequency of promoter methylation of RASSFof RASSF1A gene was 39. 3% . It was not found the promoter methylation in normal brain tissues and U251 cell line. In 11 patients with the aberrant promoter methylation, five showed the gene inactivation (P = 0. 022). Conclusion Aberrant promoter methylation was found in glioma. Although the gene inactivation of RASSF1 A was not so common in glioma, its expression was lower in glioma than in normal brain tissues. Promoter methylation may contribute to the low level or loss of RASSFT A mRNA expression.
