The role of signal transduction molecule PLC in streptococcus pneumoniae inducing filamentous actin rearrangement in type n pneumocytes in vitro
- VernacularTitle:磷脂酶C信号转导分子在肺炎链球菌触发人肺Ⅱ型上皮细胞F-actin细胞骨架重排中的作用
- Author:
Banglao XU
;
Rong WANG
;
Yibing YIN
;
Gefei KANG
- Publication Type:Journal Article
- Keywords:
Streptococcus pneumoniae;
Epithelial cells;
Phototransduction
- From:
Chinese Journal of Laboratory Medicine
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether S. pn can provoke filamentous actin (F-actin) rearrangements in vitro , which will further lead to S. pn invasion of A549 and the relationship between PLC signaling molecule and. the invasion events. Methods Labelled F-actin with FITC-phalloidin, we observed F-actin rearrangements by S. pn adhesion of type Ⅱ pneumocytes ( A549). S. pn invasion of A549 cells was determined by pretreating A549 cells with Cytochalasin D . To investigate whether F-actin rearrangements can be blocked by PLC inhibitor, A549 cells were pretreated with PLC inhibitors U73122. Results Intact S. pn can promote F-actin rearrangements. Cytochalasin D is able to prevent S. pn invasion of A549 cells. Inhibitors of PLC signal transduction molecules block F-actin rearrangements dose dependently. Conclusion S. pn can provoke F-actin rearrangements through PLC signaling pathways, which will further lead to S. pn invasion of A549 cells.
