Preparation,optimization and characterization of NC-1900 loaded MePEG-PLA nanoparticles
- VernacularTitle:载NC-1900 MePEG-PLA纳米粒的处方设计、优化及表征
- Author:
Kaili HU
;
Yueling XIE
;
Wei LU
;
Xinguo JIANG
- Publication Type:Journal Article
- Keywords:
double emulsion/solvent evaporation method;
arginine-vasopressin fragment analog (NC-1900);
MePEG-PLA;
nanoparticle
- From:
Fudan University Journal of Medical Sciences
2007;34(6):800-805,820
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To optimize the preparation of NC-1900 loaded MePEG-PLA nanoparticles (NPs). Methods MePEG-PLA copolymers of different molecular weight synthesized by solvent poly-merization method were used to prepare NC-1900 loaded MePEG-PLA NPs by double emulsion/solvent evaporation method.Orthogonal experimental design and multiple regressions were used to optimize the preparation method with nanoparticle size and NC-1900 encapsulation efficiency (EE) as res-ponse variables.NPs were characterized by particle size and Zeta potential detector and transmission electron microscope.The leakage of NC-1900 from NPs was evaluated by high-performance liquid chromatography (HPLC) detection. Results MePEG3000-PLA44800 NPs prepared according to the optimized conditions had a mean diameter of (77 ± 11) nm and EE of (21.4 ± 0.1) %.Only 5% and 15% of NC-1900 were leaked in pH 7.4 PBS and blank plasma at the end of 48 h,respectively. Conc-lusions The optimized MePEG3000-PLA44800 NPs is a favorable carrier for NC-1900.
