Study on the preparation and properties of glycosaminoglycans from the ink of Sepiella maindroni de Rochebrune
- VernacularTitle:乌贼墨糖胺聚糖的制备与理化性质研究
- Author:
Xiaodong LI
;
Fengshan WANG
;
Yunsheng SONG
- Publication Type:Journal Article
- Keywords:
ink of Sepiella maindroni de Rochebrune;
glycosaminoglycans;
preparation;
physical and chemical properties
- From:
Chinese Journal of Marine Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
One kind of glycosaminoglycans (GAG) was extracted and purified from the ink of Sepiella maindroni de Rochebrune by using the procedures of trypsin and pronase digestion, ethanol precipitation and DEAE-cellulose inon-exchange chromatography. It was proved to be homogeneous by polyacrylamide gel and cellulose acetate membrane electrophoresis. No protein absorption was shown in UV spectrum, but typical absorption of GAG was shown in the IR spectrum. It was identified by paper and thin layer chromatography of the acid hydrolyzate that the GAG was composed of galactosamine, glucuronic acid and fucose. The percentage content of galactosamine, glucuronic acid, fucose, sulfate and protein in the GAG was 25. 6%, 29.2%, 23.3%, 8.2% and 3. 5%, respectively.
