Effects of tributyltin chloride on superoxide dismutase from Tegillarca granosa
- VernacularTitle:氯化三丁基锡对泥蚶SOD的影响
- Author:
Xiang XIAO
;
Yali HAN
;
Huixiang YUAN
;
Kebo LIN
- Publication Type:Journal Article
- Keywords:
Tegillarca granosa (Linnaeus);
tributyltin chloride;
superoxide dismutase (SOD)
- From:
Chinese Journal of Marine Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Superoxide dismutase(SOD) was purified from Tegillarca granosa and the effects of tributyltin chloride(TBTCl) on the enzyme activity of the SOD were analyzed at different conditios. The results showed that the effects of TBTCl on SOD activity were more ohvious at room temperature than at four degrees centigrade. 58. 1 % of the enzyme activity was reserved after SOD was incubated with 200?g?mL-1 of TBTC1 for 72 hours at room temperature, while 90. 9% of the enzyme activity was reserved at four degrees centigrade. The enzyme activity of SOD was reduced with the increase of TBTC1 concentration and interaction time. TBTCl could reduce heat-resisting, acid-resisting and alkali-resisting abilities of SOD. With TBTCl concentration increase,SOD isozyme bands in the graph of polyacrylamide gel electrophoresis were getting weaker.
