Screening of cDNA Clone for Putative RNA Polymerase Subunit of Cysticercus cellulosae
- VernacularTitle:猪囊尾蚴RNA聚合酶亚基基因的筛选及结构初探
- Author:
Xuenong LUO
;
Yadong ZHENG
;
Yongxi DOU
;
Junlin HOU
;
Zhizhong JING
;
Xuepeng CAI
- Publication Type:Journal Article
- Keywords:
Cysticercus cellulosae;
RNA, Spliced Leader RNA;
Gene Library;
DNA-Directed RNA Polymerase
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain related genes of Cysticercus cellulosae from spliced leader (SL) cDNA library. Methods Spliced leader library of Cysticercus cellulosae was constructed using SL specific primer and oligo (dT)15 with M13M4 primer, and positive clones were then screened randomly, identified with enzyme restriction, followed by sequencing and homologous analysis. Results The amino acid sequence, encoded by the positive clone with a poly (A) 22 tail and a complete open reading frame (ORF), was with homology of RNA polymerase subunit genes of human, B. napus, fission yeast, A. thaliana, C. elegans and fruit fly up to 71.6%. Conclusion The protein, RNA polymerase subunit encoded putatively by the clone, is high conservative in different species.
