Screening of antimicrobial peptide from marine clamworm Marphysa sanguinea and preliminary research on its antitumor effect
- VernacularTitle:海洋岩虫抗菌肽筛选及抗癌活性的初步研究
- Author:
Weidong PAN
;
Xianghui LIU
;
Feng GE
- Publication Type:Journal Article
- Keywords:
antimicrobial peptide;
Marphysa sanguinea;
column chromatography;
liver cancer cells;
osteoblast;
MTS/PMS assay
- From:
Chinese Journal of Marine Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Active fractions were obtained from the c(?)amworm homogenate by reverse-phase concentration, gel filtration and affinity column chromatography. The MTS/PMS assay was used in the screening of antimicrobial peptide in vitro. The MTS/PMS assay and ELISAAFP method were combined to evaluate the effects of antimicrobial peptide on the proliferation and ?-fetoprotein secretion of HepG2 cells as well as on the proliferation of normal mouse osteoblast MC3T3-E1. The results showed that the purified antimicrobial peptide from clamworm Murphysa sanguinea was a constitutive basic protein with MW 8.1 kDa and pI 8.6, which showed inhibition effects on the proliferation and ?-fetoprotein secretion of HepG2 cells at different levels. These effects have a positive correlation with the concentrations of antimicrobial peptide. No obvious inhibition and damage effects were observed on the normal osteoblast.
