Determination of circulating HBV specific CD8~+ cells by tetramer staining flow cytometry
- VernacularTitle:组织相容性抗原-肽四聚体流式细胞技术检测乙型肝炎患者特异性CD8~+T细胞
- Author:
Yu CHEN
;
Lanjuan LI
;
Bin LOU
;
Chenhuai XU
;
Xichao GUO
- Publication Type:Journal Article
- Keywords:
HLA-A2 Agtigen;
Flow cytometry;
Hepatitis B;
CD8-Positive T-Lymphocytes
- From:
Chinese Journal of Laboratory Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinical application of HLA-peptides tetramer staining flow cytometry for determining HBV specific CD8+ cells.Methods HBV specific CD8+ cells in whole blood samples of chronic hepatitis B patients were stained with tetramer complex of HLA-A2 and HBV core 18-27 peptide and counted by flow cytometry. Results The medians of percentages of HBV specific CD8+ cells of total CD8+ cells were 0.20%(0.02%～2.04%) in 11 acute hepatitis B patients and 0.05%(
