Preparation and Identification of Monoclonal Antibodies against the Region II~+ Motif in Circumsporozoite Protein of Plasmodium falciparum
- VernacularTitle:抗环子孢子蛋白保守II~+区单克隆抗体的制备及鉴定
- Author:
Ruijuan ZHANG
;
Huaimin ZHU
;
Xiangyu LI
- Publication Type:Journal Article
- Keywords:
Plasmodium;
monoclonal antibody;
circumsporozoite protein;
Region II~+
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop and identify the monoclonal antibodies (McAbs) against Region II~+ motif in circumsporozoite protein of Plasmodium falciparum. Methods BALB/c mice were immunized with 12 peptides within Region Ⅱ~+ in circumsporozoite protein of P. falciparum. Spleen cells isolated from the immunized mice were fused with myeloma cell. After three times screening with ELISA, 3 positive hybridoma cell lines were obtained. Results ELISA test indicated that the McAbs reacted with recombinant circumsporozoite protein fragment containing tandemly repeat region and conserved Region II~+. IFA test showed that the McAbs recognized not only the sporozoites of P. falciparum, but also the sporozoifes of P. yoelii. Conclusion McAbs obtained can probe the Region II~+ motif in circumsporozoite protein of P. falciparum, which might also recognize that of other Plasmodium species.
