The functional proteome preliminary study of rat arterial smooth muscle cells promoted by homocysteine
- VernacularTitle:同型半胱氨酸刺激大鼠动脉平滑肌细胞增生功能蛋白质组的初步研究
- Author:
Liyan CUI
;
Jie ZHANG
;
Tiancheng WANG
- Publication Type:Journal Article
- Keywords:
Homocysteine;
Proteome;
Two-dimensional electrophoresis
- From:
Journal of Medical Postgraduates
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:We investigated the changes of the proteins in the rat arterial smooth muscle cells induced by homocysteine.Detected the mechanism by which homocysteinemia leads to atherosclerosis. Methods:The rat arterial smooth muscle cells of primary culture were planned to be the experimental systems.Extracted the proteins from the smooth muscle cells which were induced by homocysteine and control.After 2-D electrophoresis,the map was analyzed by imaging systems. Results:After cells promoted by homocysteine, twenty-eight proteins of the smooth muscle cells changed.Twelve proteins were found to differ in quantity(11 higher and 1 lower).Sixteen proteins were seen to be unique in one region or the others(12 points of protein appear after Hcy stimulating,4 points of protein disapear after Hcy stimulating). Conclusion:The protein of rat arterial muscle cells changed after promoted by homocysteine.We considered that the proteins were related to the proliferation of the smooth muscle cells.
