The microhistological and quantification study of free gracilis muscle transplantation in treatment of the brachial plexus injury
10.3760/cma.j.issn.1001-2036.2012.06.010
- VernacularTitle:游离股薄肌移植在臂丛损伤治疗中的显微组织学及定量研究
- Author:
Xiaogang LIN
;
Liqiang GU
- Publication Type:Journal Article
- Keywords:
Gracilis;
Brachial plexus;
Nerves;
Histology;
Transplantation
- From:
Chinese Journal of Microsurgery
2012;(6):471-474,后插6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To provide histology base for the microsurgical repair of the free gracilis muscle transplantation after brachial plexus.Methods Totally 6 fresh male adult cadaveric inferior extremities were obtained.The gracilis muscle nerve were exposed and divided with the microdissection.Specimens were got from different segment after marked direction.All specimens was faced in 4％ formaldehyde solution and then crossing sections were cut by cryoultramicrotome.All slides were stained use the technique of Kamovsky-Roots AchE histochemical.The result of never tissue staining and the distribution of individual functional fascicular group were observed on each cross-section.According to the result of staining combined with the microdissection and the order of different branches branching off the nerve trunk,the distribution of individual functional fascicular group were observed on each cross-section.The 5 μm-thick routine waxed crossing sections were made and stained according to the myelin technique of Loyez.These histological sections were analyzed by using image analysis system.For each histological section,the number of the medullated nerve fibers and the section areas of the each nerve tracts and trunks were measured and calculated.Then the proportion of nerve tracts and connective tissue were calculated.The proportion of each connective tissue was adopting paired-samples t test.Results Under low power lens most of the gracilis muscle nerve were positive reaction,only a few sparse block-shape enzyme staining regions were shown.Under high power lens,the gracilis muscle nerve have clear outline,enzyme staining limited at neuraxis,no staining at myelin sheath and connective tissue.Quantitative analysis shows that the total myelinated fiberscilis nerve was about (1958 ± 375) radix.The branches arising from the posterior subdivision were more than that from the anterior (P =0.000).There were statistical difference between the number of the medullated nerve fibers and the section areas of the anterior and posterior subdivisions,the posterior subdivision were more than that of the anterior (P ＜ 0.05).There also had statistical difference between different section areas of the connective tissue in the gracilis nerve trunk (P ＜ 0.05),the section areas of the connective tissue of the distal were more than that of proximate.Conclusions The motor fascicles characteristic of the gracilis muscle nerve can be distinguished clearly by using AchE histochemical staining,combin with the microanatomy results,we can gain the distribution of the fascicular groups on each crossing sections.Using Loyez staining and quantitative analysis,we can ensure the number of the medullated nerve fibers.It is helpful to select the suitable donor nerve and ensure the dialyneury matching each other.
