The Antitumor Effects of Tumor Vaccine with Expression of Granulocyte-Macrophage Colony-Stimulating Factor Anchored on Mouse Melanoma Tumor Cell Surface
- VernacularTitle:锚定表达GM-CSF疫苗的抑瘤效果研究
- Author:
Shaohui CHENG
;
Xiaohui MA
;
Xing CHEN
;
Jiyun YU
- Publication Type:Journal Article
- Keywords:
membrance expression;
mGM-CSF;
antitumor effects
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the vaccine potency of GM-CSF anchored B16 tumor cells. Methods: In this study, mGM-CSF was expressed on surface of B16 mice melanoma cells by GPI-modifying. C57BL/6 mice were inoculated with GM-CSF anchored cells and wide-type B16 cells to evaluate whether the GM-CSF anchored cells could elicit a protective and systemtic antitumor response. Results: GM-CSF anchored cells resulted in remarkable loss of tumorgenicity in syngenetic mice. The tumor occurrence rate of GM-CSF anchored B16 cells was 58. 8% on C57BL/6 mice with 1 ? 106 B16 cells/mice inoculated ( n = 12) and that of wide-type B16 cells was 100% , The C57BL/6 mice receiving inoculation with 5 ? 105GM-CSF anchored cells/mice never grew tumor. These mice were challenged with wide-type B16 cells, and only a minority of mice grew tumor after wide-type B16 cells inoculation. Conclusion:GM-CSF protein anchored cells could elicit a protective and systemtic antitumor responses.
