A Modified Method for Transfection to SKOV3 Cells with Protamine-DOT-AP/Chol Liposome-Oligodeoxynuleotides Complex
- VernacularTitle:改良鱼精蛋白-脂质体-寡聚核苷酸复合体法转染卵巢癌SKOV3细胞
- Author:
Jianping LU
;
Hong SUN
;
Changchun CAO
;
Zhouluo OU
- Publication Type:Journal Article
- Keywords:
protamine-liposome-oligodeoxynuleotides transfer;
ovarian neoplasms;
tumor cells
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the transfer efficiency of protamine-DOTAP/Chol liposome-oligodeoxynuleotides complex to SKOV3 cells. Methods; Synthesized NF-?B decoy ODNs or scrambled ODNs labeled by FITC on the 5'ends was mixed with rationic DOTAP/cholesteral liposome and protamine, making the charge ratio of positive: negative 4. SK-OV3 cells were transfected by the complex (1 ?mol/L) and the efficiency were measured by flow cytometry ( FCM) and fluorescence meter. The direct effect of NF-KB decoy ODNs transfer on the growth of SKOV3 cell line in vitro was measured by MTT method. Results: The efficiency of LPD transfer to SKOV3 cell line was 96. 41% , significantly higher than that of naked DNA transfer which was 12. 86%. The growth of SKOV3 cell line was not affected by 48h incubation after NF-KB decoy ODNs or scrambled ODNs transfection. Conclusion: LPD method is the one with high transfer efficiency not affected by NBS( newborn bovine serum) with possibility of use in vivo.
