Invasive ductal carcinoma of the breast is prevalence of loss of heterozygosity at 8p22,11p15 and 17p13
- VernacularTitle:乳腺浸润性导管癌染色体8p22、11p15、17p13区杂合性缺失的研究
- Author:
Zhigang LI
;
Xiaojun ZHOU
;
Qingyan KONG
;
Long YI
;
Ku MENG
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Loss of heterozygosity;
Chromosoma
- From:
Journal of Medical Postgraduates
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the LOH at regions on chromosomal arm 8p22,11p15,17p13 and its relationship with clinicopathological parameters. Methods:Thirty-four paraffin-embedded tumor and corresponding noncancerous tissues were analysed. PCR was used to amplified three microsatellite markers D8S136,D11S988 and TP53 located at these chromosomal regions. PCR products were electrophoresed on 6%polyacrylamide gel and detected using silver staining. The P53, c-erBb-2,PR,ER status were determined by immunohistochemistry. Results:Of the three markers we studied, D8S136 was detected LOH at a frequency of 12 in 34 tumors(35.29%). D11S988 and TP53 were detected LOH at a frequency of 5 in 34 tumors(14.71%). There were no obvious associations between LOH at D11S988、TP53 and clinicopathological parameters, but the tumors with LOH at D8S136 were significant larger than that without LOH(P=0.0049). Conclusion: Invasive ductal carcinoma of the breast has a frequent LOH on chromosome 8p22. The loss or inactivation of putative tumor suppressor genes on 8p22 may contribute to the excessive growth of the tumors.
