Study on cryopreservation of cells and tissues
- VernacularTitle:冷冻保存细胞组织的初步研究
- Author:
Jing GUAN
;
Zhanfeng CUI
;
Chengyuan GONG
;
Jill URBAN
;
Xizheng ZHANG
- Publication Type:Journal Article
- Keywords:
cryopreservation;
cryopreservation agent;
articular cartilage;
bone marrow fibroblast monolayer cell;
structure and morphology
- From:
Chinese Medical Equipment Journal
1989;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Cryopreservation of bovine articular cartilage and Human Bone Marrow Fibroblast (HBMF) monolayer cells is studied in the paper. The results show that the high concentrations of Cryoprotective agents (CPA) coupled with the appearance of sucrose and high contents of 1,2-propanediol contribute to reducing the formation and growth of ice nuclei and have the potential to obtain good protective effects. High concentrations of CPA can result in great damages to cartilage and HBMF monolayer cells, which can be moderated by decreasing the temperature of adding and removal of CPA. In the frozen cartilage, which is put into liquid nitrogen without adding of any CPA, the architectures of both the chondrocytes and extracellular matrix have been severely damaged. While in the control group, the chondrocytes are intact and well preserved.
