Construction of DNA Vaccine of Trichinella spiralis Muscle Larvae and Its Expression in Mice
- VernacularTitle:旋毛虫肌幼虫核酸疫苗的构建及其在小鼠体内的表达
- Author:
Jing CUI
;
Hongwei ZHANG
;
Zhongquan WANG
;
Yonglong LI
- Publication Type:Journal Article
- Keywords:
Trichinella spiralis;
DNA vaccine;
antigens;
gene_gun technique;
eukaryotic expression
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct and express recombinant plasmid containing the structural gene encoding {Mr 31 000} antigen of Trichinella spiralis muscle larvae. MethodsThe target gene TspE1 was amplified by RT_PCR, cloned into pUC18 vector, and sub_cloned into the eukaryotic expression vector pcDNA3. BALB/c mice were immunized with the purified recombinant plasmid pcDNA3_TspE1 by gene_gun bombardment. The expression of recombinant plasmid in the skin tissue was observed by HE staining and immunohistochemical staining. Results and Conclusion The recombinant plasmid pcDNA3_TspE1 was successfully constructed and expressed in the BALB/c mice.
