Intra-cellular Signal Pathway and Synthesis of Prostaglandin E_2 During Invasion of Macrophage by Toxoplasma gondii
- VernacularTitle:弓形虫影响前列腺素E_2合成的细胞内信号调节途径
- Author:
Biwen PENG
;
Dali ZHENG
;
Xuemei JIANG
;
Jianyin LIN
;
Mingsen JIANG
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
macrophage;
signal pathway;
prostaglandin E-2;
arachidonic acid;
cyclooxygenase_2(COX_2)
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the signal transduction pathway of arachidonic acid(AA) and prostaglandin E-2(PGE-2) synthesis in macrophage invaded by Toxoplasma gondii. Methods Synthesis of AA and PGE-2, expression of COX_2 mRNA and protein following stimulation infection by Toxoplasma gondii were evaluated in RAW264^7 cells by ELISA, RT_PCR and Western blotting after treatment with calcium channel blocker verapamil, chelator of extracellular calcium EGTA and inhibitor of CaM trifluoperazine (TFP), selective PKC inhibitor H7. Results Production of AA and PGE-2 induced by tachyzoite was significantly inhibited by EGTA, TFP and BAPTA/AM, and the PGE-2 production was inhibited by H7, with a reduced expression of COX_2 mRNA and protein in a dose_dependent manner. Conclusion The parasite down_regulates macrophage functions by affecting PKC signaling pathways, and triggers a biochemical cascade whose signals ultimately conduct to the secretion of immunosuppressive molecules PGE-2.
