Experimental study on establishing tumor bearing nude mice model with different ovarian adenocarcinoma cell lines
- VernacularTitle:对不同的卵巢上皮性腺癌细胞株建立裸鼠动物模型的实验研究
- Author:
Qi LIU
;
Chunmin LIU
;
Jun OU
;
Zhengliang SHI
;
Fangqiu LI
;
Qunl SHI
- Publication Type:Journal Article
- Keywords:
Nude mice;
Tumor-bearing model;
Epithelial ovarian adenocarcinoma;
Cell lines
- From:
Journal of Medical Postgraduates
2004;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the best way of tumor-bearing nude mice model with epithelial ovarian adenocarcinoma cell lines. Methods:CAOV3 (wall-attached cell) and COC2 (suspended cell) cell lines were transplanted subcutaneously to the nude mice by injection with different cell concentration or tissue block implantation. Results:The rate and time of tumor formation in the two cell lines were not related to cell concentration in the range from 5?10 6/0.2 ml to 6?10 7/0.2 ml. Time of tumor formation was earlier(especially in the manner of tissue block implantation),the successful rate of tumor formation was higher and the speed of tumor growth was relatively retarded in the nude mice bore CAOV3 cell line's tumor than that in COC2 cell line's. But once tumors were formed in nude mice bearing COC2 cell line, the mice died too fast to make experiment because of very quick speed of tumor growth. Conclusion:Wall-attached cell line, tissue block implantation with trocar may be the best way to establish tumor-bearing nude mice model in epithelial ovarian adenocarcinoma cell lines.
