Detection of Duchenne and Becker muscular dystrophy patients by DNA microarray
- VernacularTitle:DNA微阵列技术检测Duchenne型/Becker型肌营养不良患者的临床应用研究
- Author:
Wenjin DU
;
Qi WAN
;
Baoren WU
- Publication Type:Journal Article
- Keywords:
DNA microarray DMD/BMD Gene deletion
- From:
Journal of Clinical Neurology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the efficient method in detection of DMD/BMD patients.Methods 18 deletion-prone exon fragments of DMD gene were amplified via molecular cloning. They were used as probes and were spotted on the slides treated with APES and poly-lysine together by manual operation to make microarray. In addition, fragments of ?-actin were used as positive contrast and those of pUC 19/EcoR I were used as negative. 30 DMD/BMD patients were detected for deletion in DMD gene with the microarray and 5 healthy people were done as normal control. Parts of the results were compared with PCR method.Results Different exon fragment deletion of DMD/BMD gene was detected in 21 patients by DNA microarray, and 10 of them were confirmed by PCR analysis.Conclusion DNA microarray assay is a convenient ,accurate and sensitive method in diagnosis of DMD/BMD patient.
