The regulation of deferoxamine on HIF-1α expression after hypoxia-ischemia brain damage in neonatal rats
10.3969/j.issn.1000-3606.2010.03.005
- VernacularTitle:新生鼠缺氧缺血性脑损伤时铁离子螯合剂对HIF-1α的调节作用
- Author:
Lihua LI
;
Xiaojuan YIN
;
Mingxia SHANG
;
Zhichun FENG
- Publication Type:Journal Article
- Keywords:
deferoxamine;
hypoxia-inducible factor 1α;
hypoxia-ischemia brain damage;
neonatal rats
- From:
Journal of Clinical Pediatrics
2010;(3):220-222,250
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of deferoxamine(DFO)on regulating hypoxia-inducible factor 1α(HIF-1α)expression after hypoxia-ischemia brain damage(HIBD)in neonatal rats,to explore the therapeutic strategy for HIBD. Methods Postnatal day 10 SD rats were divided into four groups: hypoxia-ischemia(HI)group,DFO-treated group,normal saline(NS)-treated group,and sham operation group. HIBD model was established by the ligation of right common carotid artery following the inhalation of nitrogen-oxygen mixtures containing 92% nitrogen and 8% oxygen. DFO-treated group and NS-treated group were treated by intraperitoneal injection of DFO or NS respectively. The brains were collected at 4 h,8 h,and 24 h after hypoxia. HIF-1α protein expression was detected by Western blot analysis,and HIF-1α mRNA expression was detected by using RT-PCR at each time point. Results The synthetic level of HIF-1α protein increased significantly at 4 h,peaked at 8 h,and decreased at 24 h after HI in HI group,as well as NS-treated group. However,in DFO-treated group HIF-1α protein was peaked at 4 h,maintained higher level at 8 h and 24 h after HI. The level of HIF-1α protein was much higher in HI and DFO-treated groups than those in sham controls(P ＜ 0.05). The synthetic level of HIF-1α protein were higher in DFO-treated groups than those in HI groups at each time point(P ＜ 0.05). HIF-1α mRNA expression was higher in DFO-treated groups than those in HI groups at each time point. Conclusions DFO upregulate HIF-1α protein and mRNA expression in neonatal rats with HIBD. The peak of HIF-1α protein expression are also more advanced and lasted longer after DFO-treatment.
