Immunolocalization of the Signaling Protein l4-3-3 of Schistosoma japonicum
- VernacularTitle:日本血吸虫信号蛋白14-3-3的虫体免疫定位
- Author:
Qingzhong LIU
;
Jilong SHEN
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum Sj14-3-3, monoclonal antibody, indirect immunofluorescence, localization
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the localization of the signaling protein 14-3-3 of Schistosoma japonicum (Sj14-3-3)in the parasite. Methods Cercariae were collected from the infected Oncomelania hupensis for the infection of rabbits. Fifteen-day-old schistosomula and adult worms obtained from infected rabbits 15 and 42 days post-infection were used for frozen sections and indirect immunofluorescence staining with monoclonal antibody to rSj14-3-3. Results The results showed that the Sj14-3-3 distributed mainly in the tegument, subtegument, muscle, and parenchyma of both adult worms and 15-day-old schistosomula. Conclusion The wide distribution and large sites of Sj14-3-3 in the parasite were clearly demonstrated, which established a significant clue for further studies of biologic actions and application of 14-3-3 protein.
