Preparing the probes of cDNA microarray in detection of the hepatitis D virus with PCR products
- VernacularTitle:用PCR制备检测丁型肝炎病毒基因芯片的探针
- Author:
Zhaohui SUN
;
Wenling ZHENG
;
Bao ZHANG
;
Wenl MA
- Publication Type:Journal Article
- Keywords:
Hepatitis D virus;
PCR;
Gene probec;
cDNA microarrray
- From:
Journal of Medical Postgraduates
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To preparing the probes of cDNA microarray in detection of the Hepatitis D virus. Methods:The specific primers of PCR were designed accordion to the conserved region of HDV. Results: Sequences were aligned , and the results showed that the products of PCR amplification were the specific gene fragments of HDV. Conclusions:Using PCR amplification products to prepare gene chip probe was a quickly, simple effective method.
