Cloning and Sequencing of Cathepsin L1(FheCL1) Gene cDNA of Fasciola hepatica
- VernacularTitle:肝片吸虫组织蛋白酶L1基因cDNA序列的克隆与分析
- Author:
Ren ZHANG
;
Haiyun LI
- Publication Type:Journal Article
- Keywords:
Fasciola hepatica, cathepsin L1, DNA vaccine, cloning
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To search for a candidate DNA vaccine of Fasciola hepatica. Methods Using RT-PCR and digestion with Hind III and BamHI, Fasciola hepatica secreted cathepsin L1(FheCL1) cDNA was cloned into the expression vector pcDNA3.1. Results The cloning was successful, the cDNA sequence and its deduced amino acid sequence were analyzed. There was much difference between the cloned FheCL1 and the published one. But the first 20 residues of their amino acid sequences were the same. Conclusion The recombinant plasmid pcDNA3.1-FheCL1 may be a new type of candidate DNA vaccine candidate for Fasciola hepatica. It is possible that Fasciola hepatica presents different sub-species but their amino acid residues (1 to 20) encoded by FheCL1 might build up membrane spanning helix.
